mmp1 human elisa kit  (Thermo Fisher)

Journal: Yonsei Medical Journal

Article Title: Exploring the Safety and Efficacy of Organic Light-Emitting Diode in Skin Rejuvenation and Wound Healing

doi: 10.3349/ymj.2023.0125

Figure Lengend Snippet: Comparison of concentrations of PIP1 α1 and MMP1 and mRNA expression levels of COL1A1 , MMP1 , and MMP3 after OLED and LED light irradiation. The 6 J/cm 2 OLED light irradiation significantly induced COL1A1 mRNA expression (A) and PIP1 α1 production (D). The 6 J/cm 2 LED light irradiation significantly induced MMP1 (B) and MMP3 (C) mRNA expression and MMP1 production (E), whereas the 6 J/cm 2 OLED light irradiation significantly reduced MMP3 mRNA expression and MMP1 production. * p <0.05, ** p <0.01, *** p <0.005, independent samples t-test. PIP1 α1, pro-collagen I α1; MMP, matrix metalloproteinase; OLED, organic light-emitting diode; LED, light-emitting diode; HDF, human dermal fibroblast.

Article Snippet: After 24 h, to detect pro-collagen type I peptide α1 (PIP1 α1) and MMP1 concentrations, human PIP1 α1 SimpleStep ELISA Kit (ab210966, Abcam, Cambridge, UK) and MMP1 Human ELISA Kit (EHMMP1, Invitrogen, Waltham, MA, USA) were used according to the manufacturers’ instructions.

Techniques: Comparison, Expressing, Irradiation

Journal: Journal of Immunology Research

Article Title: Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response

doi: 10.1155/2022/9926305

Figure Lengend Snippet: Monoclonal α -CLEC5A Ab triggers a time-dependent cytokine secretion from U937 WT but not from U937 CLEC5A KO cell line. (a) PMA-differentiated WT U937 cells were transferred on culture plates previously coated with 20 μ g/mL of α -CLEC5A Ab (grey symbols) or isotype ctrl (black symbols). Cell culture supernatants harvested after 16 h (upper panel), 24 h (middle panel), and 48 h (bottom panel) were tested for the indicated cytokines with MSD or ELISA kits. (b) PMA-differentiated U937 KO cells were incubated 48 h on culture plates previously coated with 20 μ g/mL of α -CLEC5A Ab (grey symbols) or isotype ctrl (black symbols). Cell culture supernatants were tested for different cytokines with MSD or ELISA kits. Values represent the mean and SD calculated from three technical replicates/condition; ∗ p < 0.033, ∗∗ p < 0.002, and ∗∗∗ p < 0.001.

Article Snippet: ELISA kits to measure MMP1 (DY901B), MIP1-a (DY270), MIP1-b (DY271), and IL-2 (DY202) were obtained from R&D Systems.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Incubation

Journal: Journal of Immunology Research

Article Title: Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response

doi: 10.1155/2022/9926305

Figure Lengend Snippet: Antibody-mediated activation of the CLEC5A receptor on human MdM leads to a mixed cytokine response. Monocytes isolated from six donors were differentiated into M0 MdM and then transferred onto culture plates coated with 10 μ g/ml α -CLEC5A Ab or with the isotype ctrl. Cell culture supernatants were harvested 24 h later and tested for several cytokines with MSD or ELISA kits. Values represent the mean ± SD from three technical replicates per each donor. Shown is the summary result of two independent experiments with n = 6 donors; ∗ p < 0.033, ∗∗ p < 0.002, and ∗∗∗ p < 0.001.

Article Snippet: ELISA kits to measure MMP1 (DY901B), MIP1-a (DY270), MIP1-b (DY271), and IL-2 (DY202) were obtained from R&D Systems.

Techniques: Activation Assay, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response

doi: 10.1155/2022/9926305

Figure Lengend Snippet: CLEC5A receptor activation on MdM does not support autologous T cell priming. MdM generated from monocytes in the presence of M-CSF (a) or GM-CSF (b) were cocultured for 48 h with pan T cells that were purified from same donors and preactivated with 0.1 μ g/mL a-CD3 Ab. T cell-derived IL-2 secretion was measured by ELISA in cell culture supernatants. Proliferation of CD4+ (left) and CD8+ T cells (right) cocultured for 5 days with M-CSF (c) or GM-CSF (d) macrophages in the presence of 0.1 μ g/mL α -CD3 Ab was measured by flow cytometry. The cells were stained in 100 μ L FACS buffer containing 1 : 20 diluted FACS antibodies against CD4 and CD8 antigens. Values represent the mean and SD from three technical replicates per donor. Shown is the summary result of two independent experiments with six donors (a and b) and one experiment with three donors (c and d); ∗ p < 0.033, ∗∗ p < 0.002, and ∗∗∗ p < 0.001.

Article Snippet: ELISA kits to measure MMP1 (DY901B), MIP1-a (DY270), MIP1-b (DY271), and IL-2 (DY202) were obtained from R&D Systems.

Techniques: Activation Assay, Generated, Purification, Derivative Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Flow Cytometry, Staining